For over fifteen years, Denver Botanic Gardens has worked to protect the rare and endangered flora of the Rocky Mountains. Population monitoring (Demography), invasive plant research, plant-insect interaction studies, rare plant reintroductions, habitat restoration, seed collection and banking, and ex-situ conservation are a few of the methods used to conserve the biodiversity of the Rocky Mountain region.

The DBG Research Department works with many federal (BLM, USFS, NPS, BOR, EPA) and state agencies to assist in the science based management of public lands and resources. Additionally, Denver Botanic Gardens at Chatfield, located in Littleton, and Mt. Goliath on Mt. Evans, are two sites that Denver Botanic Gardens not only operates, but also uses as resources for education, research and conservation projects.